In addition, the index hopping rate in DNBSEQ TM platforms is much lower as compared to that of other platforms. When combined with the PCR-free library construction method, DNBSEQ TM also has higher SNP and indel detection accuracy compared to other platforms. These advantages dramatically improve sequencing accuracy, and have much lower duplication rates in WGS/WES applications. cPAS technology has been widely used on various sequencing platforms including BGISEQ-50, BGISEQ-500, DNBSEQ-G50, DNBSEQ-G400, DNBSEQ-T7, etc.Ĭompared to other existing sequencing platforms, DNBSEQ TM sequencing technology combines the advantages from low amplification error rates from DNBs and high density patterned arrays. However, as seasonal food insecurity is of limited. It includes: DNA single strand circularization and DNB preparation technology, Patterned Arrays, DNB loading, cPAS (combinatorial Probe Anchor Synthesis), Pair-End Sequencing technology on DNBs, fluidics and imaging systems, base calling algorithms, etc. It is similar to chronic food insecurity as it is usually predictable and follows a sequence of known events. In Figure 4, the sequence GGTCAA was typed in the Find Sequence window and the Return key pressed. Another way to find a specific base in FinchTV is to enter a sequence that is located near or contains your base. This information is then used to determine the DNA sequence of the sample.Īll sequencing technologies relating to DNBs are part of DNBSEQ TM. Experiment by selecting a base number in your sequence. Experiment by selecting a base number in your sequence. The images are then converted into a digital signal using MGI's propriety software. Images are taken after the fluorescently labeled probes on the DNB are excited with lasers. The sequencing reaction starts by pumping sequencing reagents containing fluorescently labeled dNTP probes and DNA polymerase. ![]() Sequencing primer is then added and hybridized to the adaptor region of the DNB. DNBs (DNA nanoballs) are pumped with by the fluidics system and loaded onto a Patterned Array chip. MGI's DNA sequencing instruments utilize the state-of-the-art core technology called DNBSEQ TM.
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